![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 26, Issue 3, 272-277, March 1998
Department of Drug Metabolism, Central Research, Pfizer
Incorporated
The tissue distribution of two 14C drugs
were quantitatively compared using the techniques of whole body
autoradioluminography (WBAL) and radiometry. Quantitative analysis of
tissue radioactivity in whole body cryosections was accomplished from
storage phosphor images using the MicroComputer Imaging Device. After
obtaining whole body sections from four frozen rats and three frozen
ferrets, each WBAL-sectioned specimen was partially thawed before
obtaining tissue samples for radiometric analysis. For all tissues
examined, concentrations of radioactivity determined by WBAL were
comparable with those determined by dissection and liquid scintillation
analysis (DLSA), except for renal tissue obtained from different
kidneys of the same ferret and for rat ocular tissues. A 2-fold
difference was observed between WBAL and DLSA evaluations of
radioactivity in the contralateral kidneys of one ferret. DLSA
evaluation only provided an assessment of total radioactivity in the
eye, whereas WBAL evaluation determined the selective distribution of
radioactivity to ocular tissues. Resolution in DLSA evaluation of
ocular tissues was restricted by limitations of the dissection
procedure. These results indicated that the quantitation of tissue
radioactivity by WBAL was as precise as DLSA evaluation, and WBAL also
provided results to the quantitative distribution of radioactivity to
localized sites in organs not feasible by DLSA.
This article has been cited by other articles:
|
|
 |
|
  C. Prakash, K. A. Johnson, C. M. Schroeder, and M. J. Potchoiba Metabolism, Distribution, and Excretion of a Next Generation Selective Estrogen Receptor Modulator, Lasofoxifene, in Rats and Monkeys Drug Metab. Dispos., September 1, 2008; 36(9): 1753 - 1769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Dalvie, T. Cosker, T. Boyden, S. Zhou, C. Schroeder, and M. J. Potchoiba Metabolism Distribution and Excretion of a Matrix Metalloproteinase-13 Inhibitor, 4-[4-(4-Fluorophenoxy)-benzenesulfonylamino]tetrahydropyran-4-carboxylic Acid Hydroxyamide (CP-544439), in Rats and Dogs: Assessment of the Metabolic Profile of CP-544439 in Plasma and Urine of Humans Drug Metab. Dispos., September 1, 2008; 36(9): 1869 - 1883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Prakash, D. Cui, M. J. Potchoiba, and T. Butler Metabolism, Distribution and Excretion of a Selective N-Methyl-D-Aspartate Receptor Antagonist, Traxoprodil, in Rats and Dogs Drug Metab. Dispos., August 1, 2007; 35(8): 1350 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Khojasteh-Bakht, J. P. O'Donnell, H. G. Fouda, and M. J. Potchoiba METABOLISM, PHARMACOKINETICS, TISSUE DISTRIBUTION, AND EXCRETION OF [14C]CP-424391 IN RATS Drug Metab. Dispos., January 1, 2005; 33(1): 190 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Potchoiba and M. R. Nocerini UTILITY OF WHOLE-BODY AUTORADIOLUMINOGRAPHY IN DRUG DISCOVERY FOR THE QUANTIFICATION OF TRITIUM-LABELED DRUG CANDIDATES Drug Metab. Dispos., October 1, 2004; 32(10): 1190 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Solon, S. K. Balani, G. Luo, T. J. Yang, P. J. Haines, L. Wang, T. Demond, S. Diamond, D. D. Christ, L.-S. Gan, et al. Interaction of Ritonavir on Tissue Distribution of a [14C]L-Valinamide, a Potent Human Immunodeficiency Virus-1 Protease Inhibitor, in Rats Using Quantitative Whole-Body Autoradiography Drug Metab. Dispos., November 1, 2002; 30(11): 1164 - 1169. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
