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Vol. 25, Issue 11, 1234-1241, 1997

Heterologous Expression of Human Drug-metabolizing Enzymes

F. Peter Guengerich, Asit Parikh, Eric F. Johnson, Toby H. Richardson, Claes von Wachenfeldt, Jose Cosme, Frank Jung, Christian P. Strassburg, Michael P. Manns, Robert H. Tukey, Mike Pritchard, Sylvie Fournel-Gigleux, and Brian Burchell

Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine (F.P.G., A.P.), Department of Molecular and Experimental Medicine NX-4, The Scripps Research Institute (E.F.J., T.H.R., C.v.W., J.C., F.J.), Department of Pharmacology, University of California San Diego Cancer Center (C.P.S., R.H.T.), Department of Gastroenterology and Hepatology, Medizinische Hochschule Hannover (M.P.M.), URA CNRS 1288 Physiopathologie et Pharmacologie Articulaires, Faculté de Medicine, BP184-5405 Vandoevre lés Nancy (S.F-G.), and Biomedical Research Centre (M.P.) and Department of Molecular and Cellular Pathology, Ninewells Medical School, The University Dundee (B.B.)

This article is a report on a symposium held at the March 1997 meeting of the American Society for Pharmacology and Experimental Therapeutics in San Diego. Current developments in the heterologous expression of cytochrome P450, NADPH-cytochrome P450 reductase, glutathione transferase, and UDP-glucuronosyltransferase enzymes are described. Systems include bacteria, insect cells, and transient and stable mammalian cells. Uses of the products are described for discernment of which enzymes are involved in metabolism of drugs, genotoxicity assays, mutagenesis (for structure-activity relationships), large scale production of enzyme products, antibody production, and production of proteins for biophysical studies.

Copyright © 1997 by The American Society for Pharmacology and Experimental Therapeutics


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Copyright © 1997 by the American Society for Pharmacology and Experimental Therapeutics.